LaDecode: decoding mutations from long-read sequencing data of pooled amplicons.

LaDecode is a dedicated tool for decoding multiple and complex mutations induced by multiplex target-site editing within a certain region by long-read TGS of long-range PCR amplicons, including PacBio and Nanopore sequencing. For testing the program, click download to obtain the test files.

 Input files

Step 1: input reference sequence in fasta format

Or input a reference file:

Step 2: input target sequence file (optional):

Step 3: input sequencing file (in BAM or FASTA format)

Sequencing file:
Single sample (not a pooled amplicon)

Step 4: threshold setting :

(0.001-1.00)

 Barcode settings

Barcode type:   96xn barcode    12x8 barcode
 Using default barcodes of LaDecode

Select position barcode:

Select button

Select plate barcode:

Select button

 Defined your barcode

	Upload position barcode file:			Upload plate barcode file:
	Example_position_barcode.txt			Example_plate_barcode.txt

	Your email (optional):
	*In case the webpage is closed during the program's execution, it is recommended to enter a valid email address to receive the analysis results.